Hansatech 葉綠素螢光系統 - 脈衝調變葉綠素螢光儀
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Hansatech 葉綠素螢光系統 - 脈衝調變葉綠素螢光儀
Hansatech Pulse-Modulated Chlorophyll Fluorescence
植物進行光化學的能力是有限的,並且取決於一系列因素,包括環境條件引起的壓力。吸收的光能超過用於光化學的光能時植物必須透過非光化學過程有效地消散能量。這些過程包括熱能的發射和吸收輻射作為較長波長的紅外/遠紅外光能量的重新發射。這種光能的重新發射稱為葉綠素螢光。
儘管整個葉子系統的葉綠素螢光發射太弱而無法用肉眼觀察到,但可以從葉綠素溶液的萃取物中觀察到。葉綠素螢光峰值出現在光譜的紅色區域 (685 nm) 並延伸到紅外線區域約 800 nm。
這些過程中的每一個過程都直接競爭有限的吸收能量,此過程能量利用的任何變化都會導致其他過程的互補變化。使得葉綠素螢光能夠用作快速且可靠的非侵入性光化學探測。
葉綠素螢光儀專門設計用於檢測樣品中的葉綠素螢光發射。有幾種不同類型的葉綠素螢光儀可使用。
◎脈衝調變葉綠素螢光儀
脈衝調變葉綠素螢光儀使用複雜的電子設備將葉綠素螢光與環境光分開。該系統使用快速脈衝激發光來實現這一點,以誘導相應的脈衝螢光發射。螢光儀使用高靈敏度光電二極體來檢測與記錄脈衝螢光訊號並忽略任何非脈衝訊號。
Hansatech Pulse-Modulated Chlorophyll Fluorescence
植物進行光化學的能力是有限的,並且取決於一系列因素,包括環境條件引起的壓力。吸收的光能超過用於光化學的光能時植物必須透過非光化學過程有效地消散能量。這些過程包括熱能的發射和吸收輻射作為較長波長的紅外/遠紅外光能量的重新發射。這種光能的重新發射稱為葉綠素螢光。
儘管整個葉子系統的葉綠素螢光發射太弱而無法用肉眼觀察到,但可以從葉綠素溶液的萃取物中觀察到。葉綠素螢光峰值出現在光譜的紅色區域 (685 nm) 並延伸到紅外線區域約 800 nm。
這些過程中的每一個過程都直接競爭有限的吸收能量,此過程能量利用的任何變化都會導致其他過程的互補變化。使得葉綠素螢光能夠用作快速且可靠的非侵入性光化學探測。
葉綠素螢光儀專門設計用於檢測樣品中的葉綠素螢光發射。有幾種不同類型的葉綠素螢光儀可使用。
◎脈衝調變葉綠素螢光儀
脈衝調變葉綠素螢光儀使用複雜的電子設備將葉綠素螢光與環境光分開。該系統使用快速脈衝激發光來實現這一點,以誘導相應的脈衝螢光發射。螢光儀使用高靈敏度光電二極體來檢測與記錄脈衝螢光訊號並忽略任何非脈衝訊號。
Technical Specifications
- Suitability: Field and lab-based measurement assays
- Light sources:
- Modulation beam: Temperature compensated 594nm amber LED with 4-step frequency control (optional 470nm blue LED)
- Ultra-bright white LED: Actinic >3,500 μmol m-2 s-1, saturating >13,000 μmol m-2 s-1 at sample surface
- Far-red: 735nm LED
- Detector: PIN photodiode with >700 nm filter
- Detection method: Rapid peak-pulse tracking
- Sampling rate: Variable 10 Hz to 20 kHz depending upon instrument mode
- Electronics:
- 16-bit 165 microprocessor
- 8 A/D channels 12-bit resolution
- 4 external digital I/O lines
- Single 12-bit buffered DAC (0 mV – 4095 mV)
- Storage: 256 Kb backed up RAM storing up to 2,430 full trace or 12,850 parameter only data sets
- Display: 20 x 4 LCD display
- User-interface: 4-button keypad
- Power supply:
- Field-swappable battery system consisting of:
- 2 x field-swappable 2.0 Ahr lead acid batteries
- Mains battery charger.
- Optional FMS/PS 95V to 260V universal input mains supply
- Field-swappable battery system consisting of:
- Leafclips:
- FMS/PTL PAR/temperature leafclip featuring:
- Cosine corrected PAR sensor (0 μmol m-2 s-1 – 20,000 μmol m-2 s-1)
- Leaf temperature thermocouple (-10°C – 90°C)
- Remote trigger button and tripod mount
- FMS/LC: 10 x dark adaptation leafclips
- 2 x leafclip adapters (closed and open-faced)
- FMS/PTL PAR/temperature leafclip featuring:
- Dimensions: 180mm (L) x 100mm (D) x 100mm (H)
- Weight: 2.0 kg (including 1 battery).
FMS 2+
Technical Specifications
- Suitability: Field and lab-based measurement assays
- Light sources:
- Modulation beam: Temperature compensated 594nm amber LED with 4-step frequency control (optional 470nm blue LED)
- Ultra-bright white LED: Actinic >3,500 μmol m-2 s-1, saturating >13,000 μmol m-2 s-1 at sample surface
- Far-red: 735nm LED
- Detector: PIN photodiode with >700 nm filter
- Detection method: Rapid peak-pulse tracking
- Sampling rate: Variable 10 Hz to 20 kHz depending upon instrument mode
- Electronics:
- 16-bit 165 microprocessor
- 8 A/D channels 12-bit resolution
- 4 external digital I/O lines
- Single 12-bit buffered DAC (0 mV – 4095 mV)
- Storage: 256 Kb backed up RAM storing up to 2,430 full trace or 12,850 parameter only data sets
- Display: 20 x 4 LCD display
- User-interface: 4-button keypad
- Power supply:
- Field-swappable battery system consisting of:
- 2 x field-swappable 2.0 Ahr lead acid batteries
- Mains battery charger.
- Optional FMS/PS 95V to 260V universal input mains supply
- Field-swappable battery system consisting of:
- Leafclips:
- FMS/PTL PAR/temperature leafclip featuring:
- Cosine corrected PAR sensor (0 μmol m-2 s-1 – 20,000 μmol m-2 s-1)
- Leaf temperature thermocouple (-10°C – 90°C)
- Remote trigger button and tripod mount
- FMS/LC: 10 x dark adaptation leafclips
- 2 x leafclip adapters (closed and open-faced)
- FMS/PTL PAR/temperature leafclip featuring:
- Dimensions: 180mm (L) x 100mm (D) x 100mm (H)
- Weight: 2.0 kg (including 1 battery).